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room temperature rt  (Thermo Fisher)


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    Structured Review

    Thermo Fisher room temperature rt
    Room Temperature Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/Triton+X-100/10__1016_slash_j__mtbio__2026__103375-75-20-14
    Average 99 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Fused heterocyclic mescaline derivatives
    Article Snippet: .. These coverslips washed with calcium chloride-free and magnesium chloride-free phosphate buffered saline (PBS) pH 7.4 (Gibco, #70011-044) and fixed using paraformaldehyde (PFA) 4% in PBS (#J61899, thermoscientific) for 15 min at room temperature (RT) and washed three times with 0.01% of Triton-X100 in PBS (PBST) to remove remaining PFA and to permeabilize the cells. .. Unspecific binding sites were blocked with 5% Bovine Serum Albumin (BSA) solution (#9048-46-8, Millipore) in PBST for 60 minutes at RT.

    Article Title: N-heterocycle substituted tryptamine derivatives and methods of using
    Article Snippet: .. The coverslips washed with calcium chloride and magnesium chloride-free phosphate buffered saline (PBS) pH 7.4 (Gibco, #70011-044) and fixed in paraformaldehyde (PFA) 4% in PBS (#J61899, thermoscientific) for 15 min at room temperature (RT) and washed three times with 0.01% of Triton-X100 in PBS (PBST) to remove remaining PFA and to permeabilize the cells. .. Non-specific binding sites were blocked with 5% of Bovine Serum Albumin (BSA) solution (#9048-46-8, Millipore) in PBST for 60 minutes at RT.

    Article Title: Revealing biomechanical vulnerabilities in oral cancer cells using 3D coculture platform and low-frequency ultrasound
    Article Snippet: .. Cells were washed with phosphate-buffered saline (PBS, Gibco) and fixed in 4% paraformaldehyde (PFA, ThermoFisher Scientific) for 10 min at room temperature (RT) and permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 5 min at RT. .. Cells were washed thrice with PBS and blocked in 4% bovine serum albumin (BSA, Signa-Aldrich) for 1 h at RT before being incubated for 1 h at 37 ◦C with primary antibodies: rat anti-cytokeratin 8 (CK8, 1:300, Sigma-Aldrich, MABT329), mouse anti-α-smooth muscle actin (α-SMA, 1:400, Sigma-Aldrich, A2547), mouse anti-paxillin (1:300, BD Transduction Laboratories, 610052), rabbit polyclonal anti-myosin IIA non-muscle (1:400, Sigma, M8064), mouse anti-tropomyosin, Tm2 (1:300, DSHB, CG1).

    Concentration Assay:

    Article Title: Rapid profiling of transcription factor-cofactor interaction networks reveals principles of epigenetic regulation.
    Article Snippet: .. For each sample, 4 × 10 7 cells were cross-linked with 1% formaldehyde (final concentration) (Thermo Fisher, catalog # 033314.AP) for 10 min at room temperature (RT) with gentle rotation. ..

    Gentle:

    Article Title: Rapid profiling of transcription factor-cofactor interaction networks reveals principles of epigenetic regulation.
    Article Snippet: .. For each sample, 4 × 10 7 cells were cross-linked with 1% formaldehyde (final concentration) (Thermo Fisher, catalog # 033314.AP) for 10 min at room temperature (RT) with gentle rotation. ..

    Incubation:

    Article Title: Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture
    Article Snippet: The cell pellet was resuspended in 600 μL of PBS and transferred on a 96-well plate with a round bottom (Euroclone, #ET3196). .. The cells were centrifuged at 200 g for 5 min, resuspended in 100 μL of Fixable Viability Dye eFluor 450 (eBioscience, #65-0863-14) diluted 1:1000 in PBS and incubated at room temperature (RT) for 10 min. After three cycles of washes consisting of resuspending cells in 200 μL of FACS buffer (5% FBS – Life Technologies, #10270106 in PBS) and centrifuging at 200g for 5 min, the cells were fixed in 100 μL of 4% PFA (Thermo Scientific, #J61899.AP) at room temperature (RT) for 2 min. Then, the cells were washed three times as above with FACS buffer and permeabilized in 100 μL FACS buffer with 0.75% saponin (Sigma, #8047-15-2) and 0.1% Triton X-100 (Merck, #T8787-100mL) at RT for 40 min. After centrifuging at 200 g for 5 min, the pellet was resuspended with 50 μL of antibodies diluted in permeabilization buffer. ..

    Article Title: SARS-CoV-2 nonstructural protein 3 remodels the phosphorylation of target proteins via protein-protein interactions
    Article Snippet: Briefly, the cells were lysed in 1× passive lysis buffer (Promega) at 4°C for 1 h and then centrifuged at 18,000 × g at 4°C for 15 min. A 10% SDS-PAGE was used to load equivalent amounts of cell lysates. .. Proteins were transferred to nitrocellulose membranes and blocked with 5% skimmed milk in PBS containing 0.1% Tween 20 at room temperature (RT) for 1 h. Afterward, membranes were incubated overnight with the indicated primary antibodies at 4°C, followed by HRP-conjugated secondary antibodies at 37°C for 1 h. Finally, membranes were developed using an ECL substrate (Thermo Fisher Scientific) under a ChemiDoc MP Imaging System. ..

    FACS:

    Article Title: Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture
    Article Snippet: The cell pellet was resuspended in 600 μL of PBS and transferred on a 96-well plate with a round bottom (Euroclone, #ET3196). .. The cells were centrifuged at 200 g for 5 min, resuspended in 100 μL of Fixable Viability Dye eFluor 450 (eBioscience, #65-0863-14) diluted 1:1000 in PBS and incubated at room temperature (RT) for 10 min. After three cycles of washes consisting of resuspending cells in 200 μL of FACS buffer (5% FBS – Life Technologies, #10270106 in PBS) and centrifuging at 200g for 5 min, the cells were fixed in 100 μL of 4% PFA (Thermo Scientific, #J61899.AP) at room temperature (RT) for 2 min. Then, the cells were washed three times as above with FACS buffer and permeabilized in 100 μL FACS buffer with 0.75% saponin (Sigma, #8047-15-2) and 0.1% Triton X-100 (Merck, #T8787-100mL) at RT for 40 min. After centrifuging at 200 g for 5 min, the pellet was resuspended with 50 μL of antibodies diluted in permeabilization buffer. ..

    Imaging:

    Article Title: SARS-CoV-2 nonstructural protein 3 remodels the phosphorylation of target proteins via protein-protein interactions
    Article Snippet: Briefly, the cells were lysed in 1× passive lysis buffer (Promega) at 4°C for 1 h and then centrifuged at 18,000 × g at 4°C for 15 min. A 10% SDS-PAGE was used to load equivalent amounts of cell lysates. .. Proteins were transferred to nitrocellulose membranes and blocked with 5% skimmed milk in PBS containing 0.1% Tween 20 at room temperature (RT) for 1 h. Afterward, membranes were incubated overnight with the indicated primary antibodies at 4°C, followed by HRP-conjugated secondary antibodies at 37°C for 1 h. Finally, membranes were developed using an ECL substrate (Thermo Fisher Scientific) under a ChemiDoc MP Imaging System. ..



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